Journal: Nutrients
Article Title: The Impact of a Ketogenic Diet on Late-Stage Pancreatic Carcinogenesis in Mice: Efficacy and Safety Studies.
doi: 10.3390/nu16223919
Figure Lengend Snippet: Figure 9. Effect of a KD on markers of inflammation in the liver of KC mice. Protein levels of TLR2 and TLR4, and p-p65 analyzed by western blotting in liver lysates from 12-month-old KC mice fed a control diet (CD), high-fat diet (HFD), or ketogenic diet (KD). Image shows representative immunoblots of TLR2 and TLR4, and p-p65 and protein levels of vinculin used as a loading control. Each lane represents an individual mouse, and three mice per group are shown. Bands were quantified, and results are expressed as percentage control. * p < 0.05, data are depicted as mean with ± SD, one-way ANOVA (n = 4–6 animals/group/sex).
Article Snippet: Membranes were blocked in 5% (w/v) nonfat milk for 1 h and subsequently incubated with the following antibodies: phospho-protein kinase B (Ser473) (p-Akt) (Cat #4060, RRID: AB_2315049), protein kinase B (AKT) (Cat #9272, RRID: AB_329827), adipose triglyceride lipase (ATGL; Cat #2138, RRID: AB_2167955), phospop65 (Ser536) (p-p65) (Cat #3033, RRID:AB_331284), p65 (Cat #8242, RRID:AB_10859369), and toll-like receptor 2 (TLR2) (Cat #12276, RRID: AB_2797867) from Cell Signaling Technologies (Danvers, MA, USA); Sterol regulatory element binding protein 1 (SCREBP1) (sc-13551, RRID: AB_628282), fatty acid synthase (FAS) (sc-74540, RRID: AB_1121387), 3-hydroxymethyl-3-methylglutaryl-CoA synthase HMGCS (sc-373681,RRID: AB_10947237), peroxisome proliferator-activated receptor alpha (PPARα) (sc-398394, RRID: AB_2885073), collagen type 1 alpha 1 chain (Col1A1) (sc-59772, RRID: AB_1121787), and toll-like receptor 4 (TLR4) (sc-293072, RRID: AB_10611320) from Santa Cruz Biotechnology (Santa Cruz, CA, USA); and 3-hydroxymethyl-3-methylglutaryl-CoA lyase (HMGCL) (16898-1-AP, RRID: AB_2295304) from Proteintech Group, Inc. (Rosemont, IL, USA).
Techniques: Western Blot, Control
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